Solid phase immunoassay for C trachomatis.
نویسندگان
چکیده
منابع مشابه
Solid phase immunoassay for C trachomatis.
CORRESPONDENCE Solid phase immunoassay for C trachomatis The development of chlamydial antigen detection kits has led to the wider availability of diagnostic services for this organism. Currently, two technologies are readily available; immunofluorescence microscopy and enzyme linked immunosorbant assay (EIA). Neither technology is perfect, with problems of sensitivity and of specificity.' Adva...
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Clearview Chlamydia (Unipath Limited, Bedford, United Kingdom) is a rapid immunoassay for the direct detection of Chlamydia trachomatis antigen. This assay was evaluated against the tissue culture method by using 376 paired endocervical specimens. The Clearview assay had a sensitivity of 93.5% and a specificity of 99% when it was compared with the tissue culture method. This assay does not requ...
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We describe a solid-phase enzyme immunoassay for ferritin in human serum, with use of a horseradish peroxidase-labeled antibody and a highly sensitive chromogen, 2,2'-azino-di(3-ethyl-benzthiazoline-6-sulfonate). The assay requires only 10 microL of serum per assay, relatively less time and labor than other assys for ferritin, and as little as 10 pg of ferritin can be measured. We believe this ...
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The technical complexity of determining the serovar of Chlamydia trachomatis strains has limited the use of serotyping in clinical and epidemiologic studies. We developed a simple method for rapidly serotyping isolates of C. trachomatis by using monoclonal antibodies in a dot-enzyme-linked immunosorbent assay (ELISA) system. Isolates were passaged three to six times in shell vial cultures to gr...
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A solid-phase enzyme immunoassay is described for measuring hepatitis B surface antigen in human serum or plasma. Immunologically purified antibody labeled with horseradish peroxidase was used as the indicator. In the assay system, antibody-coated controlled-pore glass is used as a solid support and there are three sequential incubations, totaling 2 h, at room temperature. Results for serially...
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ژورنال
عنوان ژورنال: Sexually Transmitted Infections
سال: 1991
ISSN: 1368-4973
DOI: 10.1136/sti.67.3.268